Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + AMPK (A2/B1/G1) Kinase Enzyme System
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega p38 gamma Kinase Enzyme System, 1mg
Recombinant full-length human p38 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 gamma is a member of the p38 MAPK family, which is activated in response to stress.
Promega WNK1 Kinase Enzyme System, 1mg
Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ABL1 (T315I) Kinase Enzyme System, 10microg
Recombinant human ABL1 (T315I) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega DYRK2 Kinase Enzyme System, 1mg
Recombinant full-length human DYRK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega MAPKAPK2 Kinase Enzyme System, 1mg
Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase that is regulated via direct phosphorylation by p38 MAP kinase.
Promega PI3K (p110beta/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega ADP-Glo™ Kinase Assay + EGFR (L858R) Kinase Enzyme System
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay + PAK1/CDC42 Kinase Enzyme System
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega ADP-Glo™ Kinase Assay + MLK1 Kinase Enzyme System
Recombinant human mLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega ZAK Kinase Enzyme System, 10microg
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega ADP-Glo™ Kinase Assay + DDR2 Kinase Enzyme System
Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain.
Promega ADP-Glo™ Kinase Assay + PKCμ Kinase Enzyme System
Recombinant full-length human PKC mu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC mu is a member of the protein kinase C family that differs from the other PKC isoenzymes in structural and enzymatic properties.
Promega ALK6 Kinase Enzyme System, 1mg
ALK6 (also known as BMPR1B) is a member of the transmembrane serine/threonine kinase that is the member of the bone morphogenetic protein (BMP) receptor, which is closely related to the activin receptors, ACVR1 and ACVR2.
Promega FES Kinase Enzyme System, 10microg
Recombinant full-length human FES was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FES is a proto-oncogene that encodes a protein-tyrosine kinase distinct from c-Src, c-Abl and other nonreceptor tyrosine kinases.